02310nas a2200277 4500008004100000022001900041245012200060210006900182260001200251300001200263490000800275520141300283653000801696653001201704653001801716653002401734653001901758653001801777100001701795700001401812700001501826700001501841700001401856700001701870856014501887 2013 eng d aISSN 0937-982700aIdentification of forensically important Sarcophaga species (Diptera: Sarcophagidae) using the mitochondrial COI gene0 aIdentification of forensically important Sarcophaga species Dipt c03/2013 a491-5040 v1273 a

The identification of species of the forensically important genus Sarcophaga is very difficult and requires strong taxonomic expertise. In this study we sequenced the mitochondrial cytochrome c oxidase subunit I gene (COI) of 126 specimens of 56 W European Sarcophaga species. As such we established the first COI database for West European Sarcophaga. We added existing data to our database to yield a total dataset of 270 COI sequences from 99 species to evaluate the COI gene as a molecular diagnostic tool for species identification in this genus. Using two simple criteria (Best Match and Best Close Match) we showed that the identification success using a mini-barcode region of 127 bp was very low (<61%). The identification success using the standard barcode region (658 bp) or using the entire COI region (1535 bp) was similar but relatively low: 75-80%. This was mainly due to specimens that were only represented by a single sequence in the dataset. Omitting these species resulted in a strong increase of the identification success: 96.9-98.5%. This success was even further increased when applying a nucleotide-based analysis but the latter may not be suitable for decision making. Even though the COI gene seems suitable to discriminate among most Sarcophaga species, additional markers will be necessary to achieve a 100% identification success.

10acoi10adiptera10aDNA Barcoding10aForensic entomology10aidentification10aSarcophagidae1 aJordaens, K.1 aSonet, G.1 aRichet, R.1 aDupont, E.1 aBraet, Y.1 aDesmyter, S. uhttps://insectafgseag.myspecies.info/fr/content/identification-forensically-important-sarcophaga-species-diptera-sarcophagidae-using-mitocho01550nas a2200181 4500008004100000245009500041210006900136300001200205490000700217520096800224653001801192653002401210653001701234100001901251700002001270700002501290856005301315 2008 eng d00aKey to the adults of the most common forensic species of Carvalho Diptera in South America0 aKey to the adults of the most common forensic species of Carvalh a390-4060 v523 aKey to the adults of the most common forensic species of Diptera in South America. Flies (Diptera, blow flies, house flies, flesh flies, horse flies, cattle flies, deer flies, midges and mosquitoes) are among the four megadiverse insect orders. Several species quickly colonize human cadavers and are potentially useful in forensic studies. One of the major problems with carrion fly identification is the lack of taxonomists or available keys that can identify even the most common species sometimes resulting in erroneous identification. Here we present a key to the adults of 12 families of Diptera whose species are found on carrion, including human corpses. Also, a summary for the most common families of forensic importance in South America, along with a key to the most common species of Calliphoridae, Muscidae, and Fanniidae and to the genera of Sarcophagidae are provided. Drawings of the most important characters for identification are also included.10aCarrion flies10aForensic entomology10aneotropical.1 aJosĂ©, Claudio1 ade Carvalho, B.1 ade Mello-Patiu, C.A. uhttps://insectafgseag.myspecies.info/fr/node/34501290nas a2200181 4500008004100000245010700041210006900148300001200217490000700229520068900236653002400925653002500949653002300974653001300997100002401010700002101034856005301055 2009 eng d00aDiagnosis and key of the main families and species of South American Coleoptera of forensic importance0 aDiagnosis and key of the main families and species of South Amer a227-2440 v533 aDiagnosis and key of the main families and species of South American Coleoptera of forensic importance. The objective of this paper is to provide diagnosis and keys of the families and species, with illustrations of the main groups. A table of all related species recorded from South America is presented, including the substrate in which they were collected and their geographical distribution. The list comprises 221 species included in 15 families, of which 70% of the species are from Brazil. Scarabaeidae is the most diverse family with 121 species, followed by Staphylinidae with 68. Also we provide one database of Coleoptera species associated with carcasses in South America.10aForensic entomology10anecrophilous beetles10aNeotropical region10ataxonomy1 aAlmeida, LĂșcia, M.1 aMise, Kleber, M. uhttps://insectafgseag.myspecies.info/fr/node/146