02133nas a2200265 4500008004100000245015700041210006900198260001200267300001200279490000800291520134000299653000801639653000801647653000801655653000901663653001801672653001001690653002401700653002701724100001401751700001701765700001401782700001701796856005401813 2012 eng d00aWhy is the molecular identification of the forensically important blowfly species Lucilia caesar and L. illustris (family Calliphoridae) so problematic?0 aWhy is the molecular identification of the forensically importan c11/2012 a153-1590 v2233 a

Species of the fly genus Lucilia are commonly used in forensic investigations to estimate the postmortem interval (PMI). Two close-related species Lucilia caesar and L. illustris are difficult to identify. Previous studies showed that the mitochondrial cytochrome c oxidase subunit I (COI) marker could be used to identify many Lucilia species. However, mixed results were obtained for L. caesar and L. illustris due to some European specimens showing identical haplotypes. Here, we investigated 58 new European male specimens of L. illustris and L. caesar whose morphological identifications were checked and for which COI fragments were sequenced. In addition, two other mitochondrial (cytochrome c oxidase subunit II and 16S) and two nuclear (internal transcribed spacer 2 and 28S ribosomal RNA) markers were obtained for a subset of these samples. For each marker, genetic divergence within each species was in the same range as between species, confirming the close relationship between both species. Moreover, for each of the gene fragments, both species shared at least one haplotype/genotype. Hence, none of the molecular markers tested could be used, alone or in combination, to discriminate between L. illustris and L. caesar.

10a16S10a28S10acoi10aCOII10aDNA Barcoding10aITS-210aPostmortem interval10aSpecies identification1 aSonet, G.1 aJordaens, K.1 aBraet, Y.1 aDesmyter, S. uhttp://dx.doi.org/10.1016/j.forsciint.2012.08.02002310nas a2200277 4500008004100000022001900041245012200060210006900182260001200251300001200263490000800275520141300283653000801696653001201704653001801716653002401734653001901758653001801777100001701795700001401812700001501826700001501841700001401856700001701870856014501887 2013 eng d aISSN 0937-982700aIdentification of forensically important Sarcophaga species (Diptera: Sarcophagidae) using the mitochondrial COI gene0 aIdentification of forensically important Sarcophaga species Dipt c03/2013 a491-5040 v1273 a

The identification of species of the forensically important genus Sarcophaga is very difficult and requires strong taxonomic expertise. In this study we sequenced the mitochondrial cytochrome c oxidase subunit I gene (COI) of 126 specimens of 56 W European Sarcophaga species. As such we established the first COI database for West European Sarcophaga. We added existing data to our database to yield a total dataset of 270 COI sequences from 99 species to evaluate the COI gene as a molecular diagnostic tool for species identification in this genus. Using two simple criteria (Best Match and Best Close Match) we showed that the identification success using a mini-barcode region of 127 bp was very low (<61%). The identification success using the standard barcode region (658 bp) or using the entire COI region (1535 bp) was similar but relatively low: 75-80%. This was mainly due to specimens that were only represented by a single sequence in the dataset. Omitting these species resulted in a strong increase of the identification success: 96.9-98.5%. This success was even further increased when applying a nucleotide-based analysis but the latter may not be suitable for decision making. Even though the COI gene seems suitable to discriminate among most Sarcophaga species, additional markers will be necessary to achieve a 100% identification success.

10acoi10adiptera10aDNA Barcoding10aForensic entomology10aidentification10aSarcophagidae1 aJordaens, K.1 aSonet, G.1 aRichet, R.1 aDupont, E.1 aBraet, Y.1 aDesmyter, S. uhttps://insectafgseag.myspecies.info/fr/content/identification-forensically-important-sarcophaga-species-diptera-sarcophagidae-using-mitocho